基于中药质量标志物的人参、西洋参、三七及相关中成药质量控制方法研究

刘永利, 雷蓉, 王晓蕾, 赵振霞, 冯丽, 魏锋, 马双成, 刘昌孝

中国药学杂志 ›› 2019, Vol. 54 ›› Issue (17) : 1402-1410.

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中国药学杂志
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中国药学杂志 ›› 2019, Vol. 54 ›› Issue (17) : 1402-1410. DOI: 10.11669/cpj.2019.17.007
中药质量与安全专刊

基于中药质量标志物的人参、西洋参、三七及相关中成药质量控制方法研究

  • 刘永利1, 雷蓉1, 王晓蕾1, 赵振霞1, 冯丽1, 魏锋2, 马双成2, 刘昌孝3*
作者信息 +

Research on Quality Control Methods of Panax ginseng, Panax quinquefolius, Panax notoginseng and Related Proprietary Chinese Medicines Based on Q-Marker

  • LIU Yong-li1, LEI Rong1, WANG Xiao-lei1, ZHAO Zhen-xia1, FENG Li1, WEI Feng2, MA Shuang-cheng2, LIU Chang-xiao3*
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摘要

目的 基于中药质量标志物(Q-marker)的理念及研究模式,对人参、西洋参、三七质量标志物进行研究,并建立质量控制方法。方法 对人参、西洋参、三七的物质基础、生物合成途径等进行分析,用液相色谱-质谱联用法对三种药材中主要成分进行测定,探寻人参、西洋参、三七的质量标志物并建立含量测定方法。结果 人参、西洋参、三七的质量标志物为其特有成分人参皂苷Rf、拟人参皂苷F11与三七皂苷R1及共有成分人参皂苷Rg1、Re与Rb1的比例特征,分别采用超高效液相色谱法与超高效液相色谱-串联质谱联用法建立了人参、西洋参、三七药材及含人参、西洋参、三七的中成药中质量标志物的含量测定方法。结论 选择的质量标志物科学合理,可反映三种药材的特性,建立的含量测定方法提高和完善了人参、西洋参、三七药材、饮片及相关中成药的质量控制水平。

Abstract

OBJECTIVE To explore Q-markers of Panax ginseng, Panax quinquefolius, Panax notoginseng and establish the content determination method based on the concept and research model of Q-marker. METHODS The material basis and biosynthesis pathway of Panax ginseng, Panax quinquefolius, Panax notoginseng were analyzed. The main components of three medicinal materials were determined by liquid chromatography-mass spectrometry, and methods for determination of Q-markers in Panax ginseng, Panax quinquefolius, Panax notoginseng herbs and proprietary Chinese medicine were established. RESULTS The Q-markers of Panax ginseng, Panax quinquefolius, and Panax notoginseng were their specific constituents, including ginsenosides Rf, pseudo ginsenoside F11, notoginseng saponins R1, and the ratio feature of the common constituents, including ginsenosides Rg1, ginsenosides Re, and ginsenosides Rb1. METHODS for determination of Q-markers in Panax ginseng, Panax quinquefolius, Panax notoginseng herbs and proprietary Chinese medicine were established by ultra-high performance liquid chromatography (UPLC) and ultra-high performance liquid chromatography-electrospray ionization quadruple mass spectrometry (UPLC/MS/MS). CONCLUSION The Q-markers which were selected are scientific and reasonable, also can reflect the characteristics of the three medicinal materials. The established methods would improved the quality control level of Panax ginseng, Panax quinquefolius, Panax notoginseng and related proprietary Chinese medicine.

关键词

中药质量标志物 / 人参 / 西洋参 / 三七 / 质量控制

Key words

Q-marker / Panax ginseng / Panax quinquefolius / Panax notoginseng / quality control

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刘永利, 雷蓉, 王晓蕾, 赵振霞, 冯丽, 魏锋, 马双成, 刘昌孝. 基于中药质量标志物的人参、西洋参、三七及相关中成药质量控制方法研究[J]. 中国药学杂志, 2019, 54(17): 1402-1410 https://doi.org/10.11669/cpj.2019.17.007
LIU Yong-li, LEI Rong, WANG Xiao-lei, ZHAO Zhen-xia, FENG Li, WEI Feng, MA Shuang-cheng, LIU Chang-xiao. Research on Quality Control Methods of Panax ginseng, Panax quinquefolius, Panax notoginseng and Related Proprietary Chinese Medicines Based on Q-Marker[J]. Chinese Pharmaceutical Journal, 2019, 54(17): 1402-1410 https://doi.org/10.11669/cpj.2019.17.007
中图分类号： R284   

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基金

国家“重大新药创制”科技重大专项资助项目(2014ZX09304307-002)
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